Problem 2, 135

Problem 2, 135 - What conclusion can be reached about the...

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Problem 2 (Due Wednesday, Feb. 23 rd ) a) A sample contains four proteins with the following properties: P 1 : M r 12,000; pI = 10 P 3 : M r = 28,000; pI = 8 P 2 : M r 62,000; pI = 4 P 4 : M r = 9,000; pI = 5 Explaining your reasoning, predict the order of emergence of these proteins from the column when the sample is chromatographed using: i. DEAE cellulose (pH 7) as a solid support and eluting with a linear salt gradient ii. CM cellulose (pH 7) as a solid support and eluting with a linear salt gradient iii. a size exclusion column with a fractionation range of 1 kD to 30 kD
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Unformatted text preview: What conclusion can be reached about the amino acid composition of P 1 relative to that of P 2 ? b) Draw the structure of the tetrapeptide HECK in an ionization state consistent with physiological pH (7.0), and calculate its approximate isoelectric point. Use 3.65 and 7.80 as the pK a values of the C-terminal carboxyl and N-terminal amino groups, respectively, and the solution pK a ’s of the amino acid side chains in your calculation (E: 4.07, H: 6.04, C: 8.37, K: 10.54)....
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