Lab-4 lipid analysis

Lab-4 lipid analysis - Objectives Lab 4 Free fatty acids...

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Lab 4 Free fatty acids Lipid peroxide, and TBARS Understand chemistry and principles of three lipid analysis methods Carry out the experiments and write a lab report Objectives
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Triglyceride RO O O O O O O H Free fatty acid Acid lipase
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Where FFA come from ? Lipase in oil seeds Acid hydrolysis during extraction and refining Lipid oxidation during storage Peroxide → aldehyde → acid High FFA = poor quality
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Analyze Free Fatty Acids Similar to a Titratable Acidity. Dealing with an oil sample makes analysis more difficult, because some oils are colored. pH meters and oil don’t get along very well, so you must use an indicator and try your best. Sample dissolved in ethanol and titrated until the fatty acids are neutralized (~pH 8.2).
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O RO O 2 O OH O RO H H O O O 2 Lipid oxidation Primary products Peroxide Secondary products malonaldehyde
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Time course of lipid oxidation
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Lab-4 lipid analysis - Objectives Lab 4 Free fatty acids...

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