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Microscope1 - TheMicroscope Whatisamicroscope...

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The Microscope
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What is a microscope? • Optical instrument the uses a lens or combination of lenses to magnify and resolve the fine detail of an object • Many different types – Magnifying glass (5‐10x) – Stereoscopic microscope – Compound microscope – Comparison microscope – Polarizing microscope – Microspectrophotometer – Scanning electron microscope (SEM) (100,000x)
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• Great disparity between potential and actual use • Range of forensic applications is broad • Used in all forensic disciplines • Extract useful information from a variety of samples – Physical characteristics • Structural information – Chemical characteristics • Identifications • Cool websites: http://www.ou.edu/research/electron/mirror http://www.microscopelab.com/microscope/ Microscope in Forensic Science
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Real Image • Formed by actual convergence of light rays on a screen (or film, or retina) • Formed on opposite side of lens and inverted
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•Image that cannot be seen directly •Seen only by viewing through lens •Image is not inverted Virtual Image
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Anatomy of a Compound Microscope Ocular or Eyepiece Illuminator Tube Stage Objective Substage Condenser Field Diaphragm Iris Diaphragm Fine and coarse focus adjustment Base Arm
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Illumination • Transmitted illumination: light passes through specimen; placed below specimen being viewed • Vertical or reflected illumination: light is reflected off of the subject; placed above the specimen – Used for opaque specimens
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Definitions • Parfocal: a unique microscope that when an image is focused with one objective in position, the other objective can be rotated into place and the field will remain in focus.
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