pGLO Transformation of E. coli A procedure known as genetic transformation has been conducted on the bacterium Escherichia coli (E. coli), strain HBIOI :K-12. This strain is unicellular, reproduces every 20 minutes in optimum conditions, does not make people sick, and it cannot survive outside the laboratory. Control Plates = (-)pGLO on LB/amp the growth medium has ampicillin in it. and (-)pGLO on LB ani.'. the growth medium has no ampicillin in it. Experimental Plates = (+)pGLO on LB/amp the growth medium has ampicillin in it and plasmid DNA was added to the bacterial cultural and T the growth medium has
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