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HW3_2011

HW3_2011 - desired glucose level in the exit stream is 0.2...

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Homework Assignment 3 126:410 Process Biotechnology Sp11 due: 2 March 2011

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Learning objectives: mass balance; linear and nonlinear equations; Solver Problem 1 The enzyme glucose oxidase is used commercially to remove glucose from dehydrated egg to improve color, ﬂavor and shelf-life. The reaction is: C 6 H 12 O 6 + O 2 + H 2 O --→ C 6 H 12 O 7 + H 2 O 2 The products are gluconic acid and hydrogen peroxide. The enzyme is immobilized on beads in a column and is not removed due to reaction. A process is set up that continuously feeds a dehydrated egg slurry con- taining 2% (w:w) glucose, 20% water and 78% egg solids to a reactor at a rate 3,000 kg/h. Air is supplied to deliver oxygen at a rate of 18 kg/h. The
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Unformatted text preview: desired glucose level in the exit stream is 0.2%. What is the composition of the oﬀ-gas and the ﬁnal egg product? Problem 2 Citric acid is manufactured in a batch fermentor using Aspergillus niger at 30 ◦ C. Over a period of two days, 2500 kg of glucose and 860 kg of oxygen are consumed to produce 1500 kg citric acid, 500 kg biomass and other products, primarily water and CO 2 . Ammonia is used as the nitrogen source. Power input to the system by mechanical agitation of the broth is about 15 kW; approximately 100 kg of water is evaporated over the culture period. Estimate the cooling requirements (kJ). 1...
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HW3_2011 - desired glucose level in the exit stream is 0.2...

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