2 Microscopy etc F10

2 Microscopy etc F10 - Microbiology 101 GENERAL...

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Microbiology 101 GENERAL MICROBIOLOGY August 30, 2010 http://www.biology.sjsu.edu/ Meet Our Team Biololgy Faculty John Boothby (Greensheets and course materials) Username: ewok\biostudents pw: 4biolecture
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Amoeba 100 μm Red blood cell 7 μm Bacterium 1 μm ('average' bacterial cell 1-2.5 μm) Virus 0.08 μm Cell sizes Concept: 1 M ============= 1 mm ========== 1 μm ====== 1 nm 1000 times smaller 1000 times smaller 1000 times smaller 1000 times smaller Calculations: 1 M ====== 10 3 mm ===== 10 6 μm ===== 10 9 nm 10 3 10 3 10 3 Cell size/scale ( m m/mm/cm/M)
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0.2 m m 0.2 mm
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Magnification (enlargement of image. ..10 X) Contrast (difference between Resolution (clarity of image) Resolving power: The minimum distance between two objects which can be distinguished as separate and distinct. Microscopy:
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Light Microscope Magnification: Simple (10X) Compound (10X x 10X = 100X) Contrast: Stain (Brightfield: Neg/Pos/Differential) Optical (Phase contrast, darkfield, etc)
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2 Microscopy etc F10 - Microbiology 101 GENERAL...

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