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Lab Report 5 - Enzymes_1

Lab Report 5 - Enzymes_1 - 1 INETLabReport5 EnzymesTemplate...

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1   INET Lab Report 5 Enzymes Template Student:    Email :   Date:    I. Enzyme Structure and Function EXERCISE 5.1  – Preparation of an enzyme activity standard 6. At five minute intervals over the next fifteen minute period, the color intensity of the solution of each test tube is  recorded ( Table 5.1 ). Time (min) Tube S 1 Potato Extract + Catechol Tube S 2 Potato Extract + Water Tube S 3 Catechol + Water 0 Yellowish - 1 Milky - 0 Clear to milky - 0 5 Not pictured in lab Not pictured in lab Not pictured in lab 10 Yellowish orange - 2 Milky - 0 Clear - 0 15 Brownish red/red-orange –  4/5 Real light brownish red- orange/orange – 3/4 Cleark to milky – 0  Table 5.1 Formation and detection of benzoquinone How can benzoquinone be detected? If it returns an intense color over x amount of time. Which two solutions need to be present to produce the brown-colored benzoquinone? To produce benzoquinone you need catechol and oxygen What substance is not present in tube S for the production of the brown-colored substance? The catechol  What substance is not present in tube S for the production of the brown-colored substance? The potato extract  II. Enzyme Specificity EXERCISE 5.2  – Enzyme specificity ©  Access Learning Systems
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2   6. At five and ten minute intervals the color intensity in each test tube is recorded. ( Table 5.2 ).
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