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Tetrad - Sample Tetrad Analysis Problem BIOL 340 The...

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Sample Tetrad Analysis Problem BIOL 340 The following 300 ordered spores were obtained from the cross between a b + X + + c (1) (2) (3) (4) (5) (6) (7) (8) a b + a b + a b + a b + a b + a b + a b + a b + + b c a + + a b + + + + a + c + + c a b c + b + a + + + b c + + c a b c + b + a b + + + + a + c + + c + + c + + c + + c + + c + + c + + c + + c 45 5 146 1 10 20 15 58 Part I: Determine map distance between each gene and its centromere. Part II: Determine which genes are linked. Part III: Determine map distance between each pair of genes. Part IV: Construct a complete map . Part V: Diagram the parental chromosomes for each tetrad at metaphase I showing minimum precise locations of crossing over to produce tetrad. Part I: First determine for each gene whether each of the 8 types of tetrads is FDS (alleles separate at meiosis I – no crossing over between gene and centromere) or SDS (alleles separate at meiosis II – crossing over between gene and centromere occurs). For gene a and its centromere: numbers # (2), (3), (5), and (7) are FDS (total = 176) and the other four types are SDS (total = 124). Therefore, MD a-centromere = 20.7.
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