RNA structure and Transcription_NR_2011(1)

RNA structure and Transcription_NR_2011(1) - RNA Structure...

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RNA Structure and synthesis CENTRAL DOGMA ??????????????????????
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RNA Structure and synthesis The information stored in DNA is copied into RNA OR DNA-directed RNA synthesis OR Synthesis of RNAs using DNA as the template TRANSCRIPTION
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RNA Structure and synthesis RNA: Ribonucleic acid Polymer of----------------------linked by-------------------- Relatively less stable ??? Function: 1. ??? 2. Regulation of gene expression Q: List out the differences between DNA and RNA
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RNA Structure and synthesis Types of RNA 1. mRNA (5%) 2. tRNA (15%) 3. rRNA (80%) 4. Other RNAs (micro RNA, hnRNA, etc.) Differ in size,function and general stability
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RNA Structure and synthesis Messenger RNA (mRNA) - Long, linear strand Describe?????????????
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RNA Structure and synthesis In eukaryotes unique chemical characters -Capping The 5’ end of mRNA is capped by 7-methyl guanosine triphosphate (speciality????) (7mGTP )
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RNA Structure and synthesis - CAP is involved in the recognition of mRNA by the translation machinery and probably helps in the stability ???? - The protein synthesis is from 5’ end or -------end (what is the complementary base on DNA for 7mGTP? - 3’ end has Poly A tail (20-250 nucleotides) - Function?? - Most heterogeneous in size and stability Function: The nucleotide sequence of an mRNA molecule contains the information that specifies the correct order of amino acids during the synthesis of a protein (the ultimate gene product)
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RNA Structure and synthesis Transfer RNA (tRNA) - Smallest, 74-95 nucleotides - 4 main arms (loops) - Acceptor arm - CCA (polarity?) - Added post transcriptionally - Amino acid is attached to ??? - Anti codon loop - D loop (Dihydro uracil) - TψC loop (pseudo uridine) - The primary structure gives rise to secondary structure - At least 20 species ??? - Function: “Adapters”
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RNA Structure and synthesis Ribosomal RNA (rRNA) - rRNA + Proteins = Ribosome (DNA + proteins = ------------) (RNA + Protein= ribonucleoprotein) eg.???? - Mammalian ribosome (eukaryotes) 60S 28S rRNA, 5.8S rRNA, 5S rRNA 80S 40S 18S rRNA - Prokaryotes 50S 5S rRNA, 23S rRNA 70S 30S 16S rRNA S ????
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RNA Structure and synthesis Functions: -Necessary for ribosomal assembly - Binding of mRNA to ribosomes and its translation - “Ribozymes” Other types RNA - Heterogeneous nuclear RNA (hnRNA) -primary transcript - Small Nuclear RNAs (Sn RNA) - mRNA processing - Gene regulation eg: U1,U2,U4,U5,U6,U7… - Micro RNA (miRNA) - Gene regulation (gene silencing) - RNA-RNA duplex - Potential target for therapeutic drug development siRNA= synthetic miRNA
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synthesis TRANSCRIPTION The nucleotide sequence “language” of DNA is precisely copied, or transcribed, into the nucleotide sequence of an RNA molecule. Requirements:
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RNA structure and Transcription_NR_2011(1) - RNA Structure...

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