Lecture 3 - Jan 26

Lecture 3 - Jan 26 - Hormones and Behavior Behavior January...

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Unformatted text preview: Hormones and Behavior Behavior January 26, 2010 Methods in Endocrinology (Continued) (Continued) Hormone Removal/Replacement – Peripheral (capsules, injections) – Central (crystalline) – Why Inject in the brain? Pharmacological techniques – Agonists & antagonists (central or peripheral) Receptor Autoradiography Receptor Electrical recording and stimulation (in vivo/in vitro) In Vitro Brain Slice In Assays: Identification and Measurement Measurement Bioassays – Based on a relatively unique biological alteration Based produced by that hormone (e.g., crop sac in doves, “rabbit test”). “rabbit Assays: Identification and Measurement Measurement Glandular size/weight – Based on a relatively unique biological alteration Based produced by that hormone (e.g., testis size; adrenal size) size) Immunoassays Immunoassays Uses principles and actions of the immune system to measure or label products or components of the neuroendocrine system. The basic principle of immunochemical techniques is that a specific antibody will combine with its specific antigen to give an antibody-antigen complex. Radioimmunoassay (RIA) Enzyme-Linked Immunosorbant Assay Enzyme-Linked (ELISA) (ELISA) Immunocytochemistry (ICC) RIA RIA ELISA ELISA Immunocytochemistry In Situ Hybridization In T-A C-G A-T G-C Reporter Genes Reporter Molecular Behavioral Endocrinology Endocrinology Quantitative PCR Quantitative – small amounts of DNA can be detected – Replaces blots for accuracy and logistics Knock Out/Mutant Animals – Clean “ablation” – May affect development and adult behavior – Testing absence of a product – Pleiotropic effects of a single gene ...
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