F09ExamIIFormAAnswer

F09ExamIIFormAAnswer - Exam II: Form A 153L FALL 2009...

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Exam II : Form A Name:_____________________ 153L FALL 2009 TA:_________Section:______ Chem 153L, FALL 2009 Exam II Cover Page December 1, 2009 TA Use Only: Be sure to: Write your name on each page. Check that there are 5 pages including the cover page. Show all work – for math problems, if strategy is not shown , then points will not be awarded! Do not skip logical steps in explaining your reasoning. But keep the answers direct. Do not repeat the question in your answer. Run-on sentences will be grammatically corrected & then graded based new sentence count. “Shotgun” answers full of keywords that do not make sense will not result in full credit. If you don’t know the answer to a question, then write what you think – don’t try to guess. Multiple choice – could have more than one right answer. Wrong answers results in deductions. If you have finished early, place your quiz face down and wait till time is called. Do not leave your seat until the quiz is done. Useful information: v o = (Vmax[S])/(Km+[S]), alpha = 1 + [I]/K I Score: P1.______P2.______P3.______ P4________P5________= _______ / 80pts.
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Exam II : Form A Name:_____________________ 153L FALL 2009 TA:_________Section:______ (+21 for page 1 problems 1-3). 1. The gel exclusion column (GEC) and SDS-PAGE are similar methods because both methods: (circle all that apply) a. separate proteins based on their size. b. rely on gravity to be the driving force for mobility of the proteins. c. involve analysis where a linear relationship can be used to relate their “velocity” to the log M r of all proteins. d. require standards and the unknown protein to be able to have variable levels of collisions with the gel matrix. e. separate proteins based on binding to the gel. Correct Answers: A, D. (+4 each worth +2, deduct +2 for incorrect choice unless zero) Incorrect Answers: B is wrong as SDS-PAGE use the force of the electric field. C is wrong because there is a limited linear range of separation. E is wrong because separation does not depend on binding to the gel, but rather collisions. 2. In SDS-PAGE, SDS functions to: (Circle all that apply) a. make all proteins have exactly the same charge to mass ratio. b. keep the protein fully denatured in its subunit form without the need for any other denaturants. c. requires practically “boiling” conditions when initially added to the protein for it to effectively work. d. must be present in all solutions of the gel and loading dye solution. e. must be rinsed out of the gel completely in order for coomassie blue to bind in order to avoid the formation of the green form. Correct Answers: C, D. (+4 each worth +2, deduct +2 for incorrect choice unless zero)
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F09ExamIIFormAAnswer - Exam II: Form A 153L FALL 2009...

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