lec 2-3 MolecularMethods - Introduction to Molecular and...

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1 Introduction to Molecular and Analytical Methods in Toxicology Common questions in toxicology • Is a gene or genes (DNA) altered by a toxicant? • Do changes in a gene result in different toxicant effects? • Does the toxicant cause changes in mRNA levels? • Which protein(s) is involved in toxicity? What experimental tools are used to answer these questions?
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2 Readings http://www.dnai.org/lesson/go/2617/ http://www.dnai.org/lesson/go/2617/16795 http://www.bio. davidson . edu /courses/genomics/method/ homolr ecomb .html http://users. rcn .com/ jkimball .ma. ultranet / BiologyPages /T/ Trans genicAnimals .html#Random_ vs ._Targeted_Gene_Insertion http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=hmg.section.484 http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=hmg.section.552 Blotting Techniques—Detection of DNA, RNA, protein targets Blotting Techniques—Detection of DNA, RNA, protein targets Biomolecules separated by electrophoresis, fixed to nylon membranes and assayed for binding to various probes - Southern Blot: Use a DNA sequence to detect DNA -Northern Blot: Use a DNA sequence to detect RNA -Western Blot: Use an antibody to detect proteins - South-Western: DNA binding to proteins or vice versa Nucleic Acid: Nucleic Acid interactions are simply due to Watson When done on a membrane we call it hybridization .
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3 Two Two copies From http://genome.ucsc.edu Mammalian Genome Structure Example from human X chromosome Chromosomal Region Exons Introns Unigene expressed sequence tag (EST) assembly
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This note was uploaded on 03/09/2011 for the course NSI 20 taught by Professor Vulpe during the Spring '11 term at University of California, Berkeley.

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lec 2-3 MolecularMethods - Introduction to Molecular and...

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