MCB+102+F10 lecture 03 - Proteins Miscellaneous low MW...

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Unformatted text preview: Proteins Miscellaneous low MW metabolites Nucleic acids Lipids Glycans 3% 60% 6% 16% 5% 10% Inorganic ions Based on data from Alberts B. et al. (2002) Molecular Biology of the Cell , 4 th Ed. Approximate Composition of a Mammalian Cell Painting from David Goodsell, The Scripps Institute A Cell Comprises Many Different Types of Proteins The Constellation of Proteins in a Cell is its "Proteome" A whole-cell lysate ("crude extract") A single pure protein PURIFICATION A complex mixture is resolved or deconvoluted into its constituents by the successive application of physicochemical methods for separation and fractionation Analysis of its AA sequence Examination of its functional characteristics Determination of its 3D structure Understanding the Function of a Protein Requires its Purification "Don't waste clean thoughts on dirty enzymes." Arthur Kornberg (1918-2007) + O O + 3 Amino acid #1 AA 3-n + Polypeptide : Every protein is a chain of amino acid residues joined by amide bonds amide bonds an amino acid "residue" Amino acid #2 + 3 the N-terminal -amino group the C-terminal ( ) carboxyl group n Characteristics of the side chains? If n is large, then the number and nature of the side chains of the AA residues in a protein dominate its overall properties R group is non-polar and aliphatic R group is aromatic R group is polar and uncharged R group is positively charged R group is negatively charged...
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MCB+102+F10 lecture 03 - Proteins Miscellaneous low MW...

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