MCB+102+F10 lecture 07

MCB+102+F10 lecture 07 - Definitions Ligand = molecule...

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Definitions Ligand = molecule (protein or otherwise) that is bound by a receptor (in our cases the receptors are proteins) in a reversible manner Prosthetic group = molecule (or ‘compound’) permanently associated with a folded protein (but it may be removed by protein denaturation) Induced fit = structural adaptation/conformational change induced by binding that increases the affinity of the binding interaction Allostery = induced fit by one subunit of a multi-subunit complex influences the conformation of the other subunit(s) Cooperativity = ligand binding by one subunit affects subunit assembly and/or subunit-ligand interaction in a multi-subunit complex
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Two views of myoglobin Ribbon depiction Cylinder depiction
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O 2 "pucker" Proximal His
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Proximal His Distal His
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Hemoglobin: The Oxygen Carrier Protein in Erythrocytes (Red Blood Cells)
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Quaternary Structure: Hemoglobin is an α 2 β 2 heterotetramer
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The Globin Fold Heme
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To measure K d , protein at limiting concentration (always less than ligand) is incubated with
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MCB+102+F10 lecture 07 - Definitions Ligand = molecule...

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