Lab #6 - LAB 6 Bacterial Transformation PHS 2301 BIOMED I...

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: LAB 6 Bacterial Transformation PHS 2301 - BIOMED I Objectives To become familiar with the structure and function of DNA To understand gene and protein expression To transform competent E. coli cells with plasmid DNA • BIOHAZARD • THE NEXT FOUR LABS INVOLVE THE USE OF BACTERIA AND DNA • WASH HANDS THROUGHLY BEFORE AND AFTER EACH LAB • CARE SHOULD BE TAKEN IN THE HANDLING OF ALL INSTRUMENTS AND DISPOSAL OF MATERIALS • DO NOT DISCARD UNTREATED BACTERIAL WASTE IN REGULAR GARBAGE AND SINKS • Transform bacteria with pGLO plasmid • Isolate DNA and Restriction digest DNA • Extract, Quantify and Separate Protein Lab Exercises 6-10 DNA DNA molecule is a polymer consisting of four different bases or nucleotides . Various combinations of these four bases create a unique DNA code (also known as genotype, gene, and allele). Nucleotides are comprised of three different components: • Nitrogen base • Deoxyribose sugar • Phosphate group Each nucleotide contains the same ribose sugar and the phosphate group. But the four nitrogen bases differ in each nucletide: Adenine (A) Thymine (T) Guanine (G) Cytosine (C) DNA nucleotide chain is created by the connection of the phosphate group to the ribose sugar of the next nucleotide . “backbone” of the DNA Biotechnology Explorer Catalog #166-2100EDU DNA Helix • Two single strands have hydrogen bonds between the nitrogen bases • The two strands are twisted together into a double helix and are: • complementary to each other- A only binds to T, and G only binds to C ( base pairing) • Run antiparallel- One strand runs 5' to 3' direction & other strand runs in 5' to 3' in the opposite direction. • Each strand carries the potential to code for genetic information • Genes are encoded in 5’ to 3’ direction • DNA molecule length is given in terms of base pairs (bp) 1 kb (kilobase pairs) = 1,000 bp 1 Mb (mega base pairs) = 1,000 kb DNA Helix •...
View Full Document

This note was uploaded on 03/09/2011 for the course PHS 2301 taught by Professor Bohu during the Spring '11 term at St. John's.

Page1 / 20

Lab #6 - LAB 6 Bacterial Transformation PHS 2301 BIOMED I...

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online