lev's lab #4 - Lab#4 Lab Report Lev Gurevich Title Enzyme...

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Lab #4 Lab Report Lev Gurevich 2-17-2011 Title: Enzyme Physical Properties Purpose/Objective : In today’s lab, we will observe substrate specificity. The enzyme α-amylase breaks down starch into smaller units of maltose, while the enzyme acid phosphatase does not. We will test to verify the ideal conditions for starch breakdown by α-amylase. Acid Phosphatase will be included in the experiment along with water as controls, as they are not supposed to break down starch. Iodine indicator will be used to test for the presence of starch. Ideally, the α-amylase enzyme under ideal conditions should show a yellow color after the indicator is added, due to the lack of iodine complex, which is formed when iodine reacts with starch. The other samples should show blue color because the conditions did not allow for the breakdown of starch. Background: Enzymes are based on substrate specificity because they bind to specific molecules, or substrates. An enzyme such as acid phosphatase, which is specific to phosphate bonds (such as in ATP or
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