Unformatted text preview: RNA and proteins have negative charge. The proteins were loaded on the cathode side, to assure that there would be protein migration. Proteins of higher net charge, lower molecular weight would move the farthest. Sources of Error : Before we added the proteins, we had to make sure that there were not air bubbles in the unit so that they would not interfere with the buffers running through the wells. Another source of error could be that not enough of the proteins sample was added in the wells or it might not have been pushed at completely. Bibliography : Class notes and powerpoint...
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This note was uploaded on 03/09/2011 for the course PHS 2301 taught by Professor Bohu during the Spring '11 term at St. John's.
- Spring '11