M- Enzyme Kinetics

M- Enzyme Kinetics - Melody Samuel-Boutros PHS 2101...

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Unformatted text preview: Melody Samuel-Boutros PHS 2101- 8:00-10:50AM Title : Enzyme Kinetics Objective : To examine the various reactions with enzymes and substrates Method : Refer to lab handout, page 13-15 Results : Phenol Assay: Tube # Stock Phenol (0.001M) Phenol Reagent Water Phenol Concentration C 1 V 1 =C 2 V 2 Absorbance 1 0.0mL 1.0mL 1.0mL blank 2 0.2mL 1.0mL 0.8mL 0.016 0.220 3 0.4mL 1.0mL 0.6mL 0.033 0.502 4 0.6mL 1.0mL 0.4mL 0.050 0.672 5 0.8mL 1.0mL 0.2mL 0.067 0.999 6 1.0mL 1.0mL 0.0mL 0.083 1.069 Determination of K m : Tube Citrate Buffer DSPP (0.005M) Water Acid Phosphatase Total Volume Conc. Of DSPP [S] C 1 V 1 =C 2 V 2 A 2.0mL 0.0mL 1.0mL 1mL 4.0mL blank B 2.0mL 0.2mL 0.8mL 1mL 4.0mL 0.0125 C 2.0mL 0.4mL 0.6mL 1mL 4.0mL 0.025 D 2.0mL 0.6mL 0.4mL 1mL 4.0mL 0.0375 E 2.0mL 0.8mL 0.2mL 1mL 4.0mL 0.05 F 2.0mL 1.0mL 0.0mL 1mL 4.0mL 0.0625 Tube # Volume of NaHCO 3 Reaction mix tube # Phenol Reagent Total Volume Final Conc. Of DSPP = [S] X 1 12 Absorbance Conc. Phenol product Obtained from graph 1 10mL A-1mL 1mL 12mL blank-.001 2 10mL B-1mL 1mL 12mL 0.00104 0.104 0.0063 3 10mL C-1mL 1mL 12mL 0.00208 0.1180....
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This note was uploaded on 03/09/2011 for the course PHS 2301 taught by Professor Bohu during the Spring '11 term at St. John's.

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M- Enzyme Kinetics - Melody Samuel-Boutros PHS 2101...

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