M- Enzyme Physical Properties

M- Enzyme Physical Properties - Melody Samuel-Boutros PHS...

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Melody Samuel-Boutros PHS 2101- 8:00-10:50 AM Title: Enzyme Physical Properties Objective : To observe and analyze the effect of temperature and pH on amylase activity Method : Refer to lab handout, page 11-12 Results : A. EFFECTS OF TEMPERATURE ON AMYLASE ACTIVITY Tube 1 : 4e C Dark blue Tube 2 : 37e C clear Tube 3 : 70 e C Medium- light blue B. EFFECTS OF pH ON AMYLASE ACTIVITY Tube 1 : 1.5mL of pH 4 buffer Dark blue Tube 2 : 1.5mL of pH 7 buffer clear Tube 3 : 1.5mL of pH 9 buffer Royal blue C. EFFECTS OF SUBSTRATE CONCENTRATION ON AMYLASE ACTIVIY Tube # 0.4% Starch PO 4 buffer Color 1 0.5mL 4.0mL Clear-yellowish 2 1.0mL 3.5mL Clear-yellowish 3 1.5mL 3.0mL Very light yellow 4 2.5mL 2.0mL Light purple 5 4.5mL 0mL Strong purple/ burghandy Discussion and References : In this lab, various physical properties of enzymes were observed by observing the effects of temperature on amylase activity, observing the effects of pH on amylase and observing their effects of substrate concentration. A catalyst
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M- Enzyme Physical Properties - Melody Samuel-Boutros PHS...

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