M- High Performance Liquid Chromatography

M- High Performance Liquid Chromatography - Melody...

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Melody Samuel-Boutros PHS 2101- 8:00-10:50 AM Title : High Performance Liquid Chromatography Objective: To understand the nature of drugs and methods of isolating these toxic substances. Methods : Refer to lab handout, page 27 Results : See attached handout. Discussion and References : In this lab we utilized three drugs, salicylic acid, Phenobarbital, and phenytoin. All three of these drugs are hydrophilic and have a great affinity for the hydrophilic mobile phase. The drugs are either weak acids or bases. Acidic drugs are unionizable in the stomach and are absorbed and basic drugs are ionized and excreted by the body. High Performance Liquid Chromatography is a popular technique for the separation of chemical substances, and is preferred because hundreds of separations maybe done using the same column, very small quantities are needed, and it is fast and accurate due to the high pressure. There are two phases in HPLC, mobile phase and stationary phase. The mobile phase is made up
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This note was uploaded on 03/09/2011 for the course PHS 2301 taught by Professor Bohu during the Spring '11 term at St. John's.

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M- High Performance Liquid Chromatography - Melody...

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