M- Quantitation of DNA by UV spectrophotometry and Plasmid restriction digest

M- Quantitation of DNA by UV spectrophotometry and Plasmid restriction digest

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Melody Samuel-Boutros PHS 2101- 8:00-10:50 AM Title: Quantitation of DNA by UV spectrophotometry and Plasmid restriction digest Objective: To ascertain the amount of DNA isolated in the previous sessions and prepare for plasmid restriction digest. Method: Refer to lab handout, page 23-24 Results: A 230 A 260 A 280 A 230/260 A 260/280 0.575 1.300 0.669 0.442 1.943 1/50ug/mL = 1.300/C unknown = 65ug/mL x 100 = 6500ug/mL/1000= 6.5 ug/mL= C initial Therefore we needed to use mL of DNA. Discussion and Reference : In this lab we ascertained the amount of DNA that was isolated and purified from last week by the spectrophotometer. We also cut the plasmid with a restriction endonuclease. This is known as a restriction digest. Spectrophotomeric methods are based on absorption. All atoms and molecules are capable of absorbing various types of energy. The amount of energy absorbed depends on the structure of the molecules and the number of molecules present. The plasmid DNA is double stranded. A ratio of OD
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This note was uploaded on 03/09/2011 for the course PHS 2301 taught by Professor Bohu during the Spring '11 term at St. John's.

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M- Quantitation of DNA by UV spectrophotometry and Plasmid restriction digest

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