M- Separation and Identification of Amino Acids by TLC

M- Separation and Identification of Amino Acids by TLC -...

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Melody Samuel-Boutros PHS 2101- 8:00- 10:50 AM Title: Separation and Identification of Amino Acids by TLC Objective : the objective of this experiment is to use thin layer Chromatography and be able to calculate the R f value and polarity of many different amino acids. Method : Refer to lab handout, page 10 Results: Amino Acid Distance traveled by AA Distance traveled by solvent R f value Glycine 3.54 cm 6.1 cm 0.58 Histidine 2.5cm 6.1 cm 0.41 Phenylalanine 4.15cm 6.1cm 0.68 Unknown 3.31cm 6.1cm 0.54 Discussion and References : Thin Layer Chromatography is a method used to determine molecules. It has a mobile phase, in which the molecule moves on the stationary phase, which is made of silica gel. The silica gel is a polar substance that absorbs solvents. Polar substances attract polar substances (“like attracts like”). The rate of absorption is dependent on the polarity of molecules. Based on polarity, the amino acid will travel at different rates. If a polar substance is on the silica gel it will travel less and absorb faster
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M- Separation and Identification of Amino Acids by TLC -...

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