REPORT#2a Absorbance of light

REPORT#2a Absorbance of light - Verina Atalla PHS 2301-...

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Verina Atalla PHS 2301- 7:30- 10:25 AM Title: Pipetting techniques and photometry Partner name: Christina L. Purpose/Objective: The purpose of this experiment is to calculate the pKa of p-nitrophenol (a weak acid) using the Beer’s Law equation and the Hnderson-Hasselbalch equation. Background: Visible light is defined as “a mixture of all the colors of light of different wavelengths”. This light range’s wavelength is from 400 - 700 nm and a color range of violet through red depending on the light at what measure it’s being portrayed. White light is a mixture of all the visible colors of light while black is the total lack of it. In order to measure this absorbance in nm, you use the following equation: Absorption (%) = Total incident light (100%) / the % transmitted light (T) Absorbance (A) = Log 100 – log %T = 2 - log %T Absorbance is measured using a spectrophotometer. You need to determine the greatest absorbance. “The spectrophotometer is more sensitive to absorbance changes at this wavelength;
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REPORT#2a Absorbance of light - Verina Atalla PHS 2301-...

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