REPORT#2b Preparation of bufferr

REPORT#2b Preparation of bufferr - Verina Atalla PHS 2301...

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Verina Atalla PHS 2301- 7:30- 10:25 AM Title: Preparation of Buffers Partner name: Christina L. Purpose/Objective: The purpose of this experiment is to use the Henderson-Hasselbalch equation in the preparation of buffers Background: A buffer is an equilibrium mixture of conjugate weak acid/base pair designed to resist change in pH. A good buffer will withstand change in pH. When making a buffer, you have to make sure that you pick a solution that has a pH close to its pKa. The closer, the better. Since phosphoric acid has many dissociation constants, it’s an easy solution to prepare. We chose the pH to be 7. Using Henderson-Hasselbalch equation, we calculated the ratio of conjugated base to acid in order to find out how much we need of each. After these calculations, we used the pH meter to verify our results Results : See graphs and tables attached Conclusion: The expected pH values for all three buffers came very close to the measured value that we obtained through experiment. This means that all our measurements were near accurate
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This note was uploaded on 03/09/2011 for the course PHS 2301 taught by Professor Bohu during the Spring '11 term at St. John's.

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REPORT#2b Preparation of bufferr - Verina Atalla PHS 2301...

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