REPORT#4 Enzyme physical properties

REPORT#4 Enzyme physical properties - Verina Atalla PHS...

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Verina Atalla PHS 2301- 7:30- 10:25 AM Title: Enzyme Kinetics Partner name: Christina L. Purpose/Objective: The purpose of this experiment is to study the enzyme kinetics of Acid Phosphatase using the substrate p-nitrophenol phosphate disodium salt (p- NPPO4Na 2 ), to determine unknown concentrations using a standard curve and to verify the Michaelis constant, K m and Maximum velocity, V max of Acid Phosphatase enzyme. Background: Enzymes are based on substrate specificity because they bind to specific molecules, or substrates. An enzyme such as acid phosphatase, which is specific to phosphate bonds will be broken down by -amylase under optimal α conditions of pH 7 and 37 degrees Celcius. Acid Phosphatase and water are in this experiment as controls. To test for the presence of starch, an Iodine indicator will be used. Ideally, the -amylase enzyme under ideal conditions should show a yellow α color after the indicator is added, due to the lack of iodine complex, which is formed when iodine reacts with starch. The other samples should show blue color because
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REPORT#4 Enzyme physical properties - Verina Atalla PHS...

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