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Unformatted text preview: Verina Atalla PHS 2301- 7:30- 10:25 AM Title: Enzyme Kinetics Partner name: Christina L. Purpose/Objective: The purpose of this experiment is to study the enzyme kinetics of Acid Phosphatase using the substrate p-nitrophenol phosphate disodium salt (p- NPPO4Na 2 ), to determine unknown concentrations using a standard curve and to verify the Michaelis constant, K m and Maximum velocity, V max of Acid Phosphatase enzyme. Background: As soon as we add the enzyme, there is an initial period of velocity increase which is dependent on the substrate concentration. When the enzyme sites start getting occupied and the substrate attaches, there comes a point where the velocity reaches its max and continues at a steady state. In order to find the km (also known as the Michaelis constant), we plot a graph of (V ) vs the enzyme substrate [S], find half of the maximum velocity and measure it down to the substrate concentration. find half of the maximum velocity and measure it down to the substrate concentration....
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This note was uploaded on 03/09/2011 for the course PHS 2301 taught by Professor Bohu during the Spring '11 term at St. John's.
- Spring '11