REPORT#6 Bacterial Transformation

REPORT#6 Bacterial Transformation - Verina Atalla PHS 2301-...

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Verina Atalla PHS 2301- 7:30- 10:25 AM Title: Bacterial Transformation Partner name: Christina L. Purpose/Objective: The purpose of this experiment is to become familiar with the structure and function of DNA, understand gene and protein expression and to transform competent E . coli cells with plasmid DNA Background: Method: Look in lab procedure manual Results : Conclusion: What purpose does a control serve? A control serves as a plate that is used as a guide to help you compare changing results to stable ones. Which plates should be compared to determine if any genetic transformation has occurred? Why? If you compare the plate with the plasmid to the one that doesn’t have the plasmid, you will determine if genetic transformation has occurred because the plasmid has an added gene that will distinguish it from the other plate Which plates should be compared to determine if any GFP gene expression has occurred? Why? The pGLO plasmid that contains the GFP gene also contains the gene for beta lactamase,
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REPORT#6 Bacterial Transformation - Verina Atalla PHS 2301-...

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