Melody Samuel-Boutros PHS 2101- 8:00-10:50 AM Title : Bacterial Transformation, Isolation of DNA Objective: To learn about the safety procedures involved with E.coli Method : Refer to lab handout, page 19-20 Results : Discussion and References : In this lab, we used E.coli which can make students sick if they get in our system. Therefore, it is essential to wash your hands before and after the experiment. We became familiar with a plasmid which is an extra chromosome piece of circular DNA, which is replicated in bacteria. Plasmids are also transferable from bacteria to bacteria. Bacterial cultures are treated so that the cells become competent to take up DNA from the media environment by the process of transformation. Plasmids are ampicillin resistant. Questions : 1. Define the difference between a nucleotide and a nucleoside. A nucleoside contains only a nitrogen base and a pentose sugar. A nucleotide has a nitrogen base bonded to a pentose sugar and phosphate group. 2. Define:
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