Lab #2 - PHS 2301 - BIOMED I LAB 2 PREPARATION OF BUFFERS...

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LAB 2 PREPARATION OF BUFFERS AND LIGHT ABSORBANCE PHS 2301 - BIOMED I
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OBJECTIVES To gain skills in Basic Laboratory techniques Care of Lab glassware and Equipment Performing Calculations Weighing and Preparing solutions To apply Henderson-Hasselbalch equation in the preparation of buffers To understand The properties of light Relationship between transmitted and absorbed light Relationship between wavelength and absorbance
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Glassware Types of glassware Types of glassware Tubes, Beakers and Erlenmeyer Flasks Volumetric Flasks and Measuring cylinders Cleaning glassware Cleaning glassware Rinse with Hot tap water - at least 3 times or until clean Cold tap water - at least 3 times Distilled water - 2 times Drain and air dry – DO NOT use paper towels Store appropriately as instructed
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Instruments Make sure you Make sure you Know how the equipment works Follow instructions for use carefully Check the units of measurement on the instrument Check the range and sensivity of the instrument Use good lab techniques Do not pour solution close to or over the instrument Make sure you clean up after you finish Remove cuvettes from spectrophotometer Rinse pH meter and place in storage solution Do not move the instruments, you may damage them
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Disposal Understand the Hazard potential of the waste created. Containers are provided at every student station for waste collection. At the end of the lab exercise the waste will be placed in appropriate containers for final disposal. Do not mix Non-Hazardous with Hazardous waste Non-Hazardous with contaminated (infectious) & biological waste Do not place pipette tips in liquid waste containers. Do not discard any Hazardous waste in the regular waste or sink. All waste must be disposed as instructed in Lab manual or by Instructor.
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Calculations Use the correct formula for calculation Make sure you have the correct units ! Concentration: Molarity (μM , mM or M ) , % (w/v or w/w) Diluted or stock solution (Ratio, dilution factor) Weight (ng, μg, mg or g) Volumes (μl, ml or L) Multiply by 1000 to convert Divide by 1000 to convert g or M milli (m) micro (μ) nano (n) Always Double check calculations
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Measuring Quantities Volume measurements: Volume measurements:
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Lab #2 - PHS 2301 - BIOMED I LAB 2 PREPARATION OF BUFFERS...

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