spectrophotometry lab

spectrophotometry lab - Molarity(µM 23.2 18.56 13.92 9.28...

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Unformatted text preview: Molarity (µM) 23.2 18.56 13.92 9.28 4.64 Absorbance (A) 0.68 0.54 0.42 0.28 0.14 Effective Molar Absor 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 0 Bromothymol Blue, 6 f(x) = 0.03x + 0.01 Absorbance (A) 5 10 15 2 Molarity (µM) Molarity (µM) 61.6 49.3 37 24.6 12.3 Absorbance (A) 0.77 0.61 0.44 0.3 0.15 Effective Molar Absor 1 0.8 0.6 Absorbance (A) 0.4 0.2 0 0 10 20 30 40 50 Molarity (µM) f(x) = 0.01x - 0.01 Thymol Blue, 434 Wavelength (nm) 370 Bromothymol Blue Absorbance (A) 0.17 Thymol Blue Unkwown Mixture B Absorbance (A) Absorbance (A) 0.48 0.34 380 390 400 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 0.19 0.21 0.21 0.21 0.17 0.14 0.12 0.11 0.11 0.11 0.11 0.12 0.13 0.16 0.19 0.22 0.25 0.3 0.36 0.42 0.47 0.54 0.61 0.66 0.67 0.61 0.5 0.35 0.22 0.22 0.53 0.58 0.65 0.65 0.75 0.77 0.76 0.73 0.67 0.59 0.5 0.42 0.33 0.26 0.21 0.17 0.14 0.13 0.13 0.13 0.14 0.14 0.15 0.13 0.1 0.07 0.05 0.03 0.01 0.01 0.38 0.42 0.46 0.48 0.5 0.51 0.49 0.47 0.43 0.38 0.33 0.28 0.24 0.21 0.18 0.18 0.18 0.19 0.21 0.23 0.26 0.28 0.31 0.33 0.31 0.27 0.21 0.14 0.09 0.05 Absorbance (A) Molar Absorbtivity ymol Blue, 618nm Absorbance (A) Linear Regression for Absorbance (A) Molarity (µM) 23.2 18.56 13.92 9.28 4.64 15 arity (µM) 20 25 Molar Absorbtivity ol Blue, 434nm Absorbance (A) Linear Regression for Absorbance (A) Molarity (µM) 61.6 49.3 37 24.6 12.3 30 40 50 60 70 larity (µM) 0.9 0.8 0.7 0.6 0.5 Bromothymol Blue 0.9 0.8 0.7 0.6 0.5 Absorbance (A) 0.4 0.3 0.2 0.1 0 370 420 470 520 570 620 670 Bromothymol Blue Absorbance (A) Thymol Blue Absorbance (A) Unkwown Mixture B Absorbance (A) Wavelength (nm) Absorbance (A) 0.13 0.1 0.07 0.04 0.02 Effective Molar Absorbtivity Bromothymol Blue, 434nm 0.14 0.12 0.1 0.08 Absorbance (A) 0.06 0.04 0.02 0 0 f(x) = 0.01x - 0.01 Absorb Linear R for Abs 5 10 15 20 25 Molarity (µM) Absorbance 0.11 0.1 0.08 0.06 0.03 Effective Molar Absorbtivity Thymol Blue, 618nm 0.12 0.1 0.08 0.06 0.04 0.02 0 0 f(x) = 0x + 0.02 R² = 0.98 Absorbance (A) Absorbanc Linear Reg Absorbanc 10 20 30 40 50 60 70 Molarity (µM) mothymol Blue mothymol Blue sorbance (A) ymol Blue Abrbance (A) kwown Mixture B sorbance (A) btivity 34nm Absorbance (A) Linear Regression for Absorbance (A) 0 25 tivity Absorbance Linear Regression for Absorbance 0 70 ...
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This note was uploaded on 03/10/2011 for the course GEOGRAPHY 101 taught by Professor Lee during the Spring '11 term at American River.

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