Ex02_S11-Analysis - Group Members Maria Bosquez Parxann...

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Group Members: Maria Bosquez, Parxann Counts, Valerie Lopez, Natalie Myers Date: 1/27/11 LI: Christina Hazard Unique: 48835 Day: TH Room: PAI 1.18 Lab Start Time 9:00 BIO206L Spring 2011 Q.# 1-10 1 point each Total 10 points Exercise 2 Analysis To be completed as a group and turned in at the beginning of your next laboratory period. Include your “Data & Results”, sketches, acquired digital images, etc. as directed by your laboratory instructor. Show your work for all calculations and/or print your MS Excel data sheets. Be sure to include proper units where necessary. Adhere to University’s Honor Code and course policies. Balance between brevity and completeness. (1) Answer the following regarding the Olympus CX31 brightfield microscope you used for this exercise. (a.) Does it have a built-in illuminator or does it have a separate illumination source? Built-in illuminator (b.) Is it a monocular, binocular, or triocular microscope? Binocular (c.) How do adjustments of the field diaphragm affect the quality of a viewed specimen? Opening the field diaphragm provides light at a wide range of angles, resulting in highest resolution, while closing it results in highest depth of field and best contrast. (d.) How do adjustments of the aperture diaphragm and light intensity affect the view of a specimen? Opening the aperture diaphragm decreases contrast by allowing a wider range of light angles to strike the specimen; however, this leads to a increase in resolution. Vice versa, closing the aperture diaphragm increases the scattering contrast but decreases resolution. (e.) How does the “field of view” change as magnification is increased? How does this relate to the fact that one should always use the low-power scanning objective first to locate a specimen? The field of view decreases as magnification increases. This is why one should find what they are looking for in a low power setting, because if they increase the magnification before they have found what they are looking for, they will have a harder time finding it after they have decreased their field of view. (f.) What happens to the “depth of focus” as magnification is increased? The lowest magnifications give us the greatest depth of focus, so this means depth of focus decreases as magnification increases. (g.) What is used to clean optical surfaces of microscopes and why? Non-abrasive lens paper since things like tissue wipes tend to be abrasive and scratch the surface of the lens (2) List the steps required to adjust the microscope for Köhler illumination. When viewing the microscope at 100X magnification, raise the condenser as far as it will go and close the field diaphragm. You should see only a small circle of light through the ocular lenses. Adjust the condenser height until the edges of this circle Spring 2011 Ex 2 Analysis Page 1 of 6
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Group Members: _____ _______________ _____________________________Exercise_______Date: ___________ LI ______________________ Unique __________ Day: __________ Room: _______ Lab Start Time ________
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