pset8-draft_3

pset8-draft_3 - Name_ Section_ TA_ 2010 7.012 Problem Set 8...

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Section_______ TA_____________ 1 2010 7.012 Problem Set 8 Please print out this problem set and answer the questions on the printout. Answers to this problem set are to be turned in at the box outside 68-120 by 1:15 pm, Friday Dec 3 rd . Question 1 You have purified a novel protein, which you call Protein R as a part of your summer project. You would like to further characterize this protein. Accordingly, you want to develop antibodies against this protein. You inject Protein R into a rabbit and after one month you draw some blood from this rabbit and determine that the rabbit’s immune system has developed antibodies against Protein R. You wait for one month and then inject Protein R again into the same rabbit. You observe a stronger secondary immune response developing within a few days against Protein R. a) On the graph below, draw the primary and secondary immune responses of the rabbit against Protein R. b) Why is the secondary immune response faster and stronger compared to the primary immune response? c) Which two classes of B cells in the immune system produce antibodies during the primary and secondary immune responses? d) The immune system’s first encounter with an antigen is via the IgM class of antibodies. Once the response is initiated, the immune cells produce the IgG class of antibodies that circulate in the blood stream. You isolate the antibodies produced against Protein R, resolve them on a protein gel based on their molecular weight and obtain a profile as shown in the schematic below. i. Identify the two classes of antibodies in lane 1 and lane 2 of the protein gel. Lane 1 of the protein gel. Lane 2 of the protein gel. ii. Do the two classes of antibodies have … identical or different antigen binding sites? same number of antigen binding sites (Yes/No)? Explain . 1
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pset8-draft_3 - Name_ Section_ TA_ 2010 7.012 Problem Set 8...

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