7.012_Section_8-_solution_key_-2010

7.012_Section_8-_solution_key_-2010 - Will not be...

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1 Solution key - 7.012 Recitation 8 - 2010 Questions: 1. You are interested in four different proteins in a yeast cell: protein 1 is a cytosolic protein, protein 2 is a secreted protein, protein 3 is a nuclear protein and protein 4 is a transmembrane protein. You plan to study how the proteins are localized to their specific destination by creating the following mutations in the genes encoding proteins 1-4. a) Mutation A inactivates the SRP (signal recognition particle). Indicate how the localization of each protein (1-4) will be affected by this mutation. In additional state whether each protein will function as it does in a wild-type cell. Protein 1: No effect Protein 2: Will not be translocated to the ER and hence will not be made. Protein 3: No effect Protein 4:
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Unformatted text preview: Will not be translocated to the ER and hence will not be made b) Mutation B prevents the fusion of vesicles to the golgi body membrane. Indicate how the localization of each protein (1-4) will be affected by this mutation. In additional state whether each protein will function as it does in a wild-type cell. Protein 1: No effect Protein 2: The protein will not be able to undergo further post-translational modifications in golgi body that its critical for its function. Note: Please note all the proteins do not undergo post-translational modification and are therefore not translocated to gogi). Protein 3: No effect Protein 4: . (Note: Please note all the proteins do not undergo post-translational modification and are therefore not translocated to gogi)....
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