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7.012_Section_9-_solution_key_-2010

7.012_Section_9-_solution_key_-2010 - Solution key 7.012...

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1 Solution key - 7.012 Recitation 9 - 2010 Questions: 1. For each part, state how many bands you would see on a gel in a lane in which you loaded: i. A circular piece of DNA that was cleaved with a restriction enzyme that cuts at one site on the circle. This will give one band on a gel. ii. A circular piece of DNA that was cleaved with a restriction enzyme that cuts at two sites on the circle those are equidistant from each other. This will generate two bands, which are of equal size. Therefore they will migrate at the same rate and will be viewed as one single band on the DNA gel. However the intensity of this band will be much higher compared to the intensity you would expect for a single band. iii. A linear piece of DNA that was cleaved with a restriction enzyme that cuts at one site on the molecule. This will generate two bands. If these bands are of different sizes they will migrate differently and appear as two bands on gel. If however they are of the same size they will appear as one band, of higher intensity on a gel. 2. Three restriction enzymes have recognition sites in a plasmid: EcoRI (“E”), HindIII (“H”), and XbaI (“X”).
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