7.012_Section_9-_solution_key_-2010

7.012_Section_9_sol - Solution key 7.012 Recitation 9 2010 Questions 1 For each part state how many bands you would see on a gel in a lane in which

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Solution key - 7.012 Recitation 9 - 2010 Questions: 1. For each part, state how many bands you would see on a gel in a lane in which you loaded: i. A circular piece of DNA that was cleaved with a restriction enzyme that cuts at one site on the circle. This will give one band on a gel. ii. A circular piece of DNA that was cleaved with a restriction enzyme that cuts at two sites on the circle those are equidistant from each other. This will generate two bands, which are of equal size. Therefore they will migrate at the same rate and will be viewed as one single band on the DNA gel. However the intensity of this band will be much higher compared to the intensity you would expect for a single band. iii. A linear piece of DNA that was cleaved with a restriction enzyme that cuts at one site on the molecule. This will generate two bands. If these bands are of different sizes they will migrate differently and appear as two bands on gel. If however they are of the same size they will appear as one band, of higher intensity on a gel. 2. Three restriction enzymes have recognition sites in a plasmid: EcoRI (“E”), HindIII (“H”), and XbaI (“X”). You digest the plasmid with each of the following combinations of enzymes and see the
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 03/12/2011 for the course BIO 7.012 taught by Professor Lander during the Fall '10 term at MIT.

Page1 / 3

7.012_Section_9_sol - Solution key 7.012 Recitation 9 2010 Questions 1 For each part state how many bands you would see on a gel in a lane in which

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online