7.012_Section_10-_solution_key_-2010

7.012_Section_10-_solution_key_-2010 - Solution key 7.012...

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1 Solution key - 7.012 Recitation 10 - 2010 Questions: 1. You are interested in making many copies of a specific DNA sequence. The sequence that you want to amplify is flanked by regions with the sequence given below: Circle the set(s) of primers that will amplify this region. 2. Take a look at the molecules drawn below #1 #2 #3 a) Which of the above molecules is non-physiological and is only used in DNA sequencing? #3 since it only has H atoms at the 2’C and 3’C position of the sugar (i.e. it has dideoxyribose sugar). b) Which one of the above molecules is used in RNA? ? #1 since it has OH groups at the 2’C and 3’C position of the sugar (i.e. it has ribose sugar). c) Which one of the above molecules is used in DNA? #2 since it has OH groups at the 3’C and H atom at 2’C position of the sugar (i.e. it has deoxyribose sugar). d) Which one of the above molecules is used as the major source of energy in cells? #1 since it is ATP, which serves as the energy currency of the cell. 3. You decide to sequence the following piece of DNA using the following primer: 5’-…GTATAGCCTCCGT…-3’ 3’-…CATATCGGAGGCA…-5’ OH HO OH Primer 1 should bind in this region Primer 2 should bind in this region
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