7.012_Section_12-solution_key-2010 - Solution key - 7.012...

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1 Solution key - 7.012 Recitation 12 - 2010 Questions: 1. Salmonella is a bacterial genus that is highly related to Escherichia , the genus of E. coli . You study a Salmonella protein that is 100% identical to an E. coli protein. i. At the nucleotide level, the Salmonella gene and the E. coli gene are only 87% identical. Explain how this is possible. There are multiple codons for the same amino acids. This difference may result in difference in gene sequence although the protein produced may still be the same. ii. Less than 5% of our genome is made up of genes. What is the other 95% made of? Most of it is regulatory elements i.e. enhancers, promoters or introns that are ultimately spliced out. iii. If you had the entire sequence of a genome of a new bacterium, how would you predict where the genes were in the genome? You could compare it by hunting for the open reading frames. iv. Why could you not use the same strategy if you had the sequence of a genome of a eukaryote? The eukaryotic genes, unlike the prokaryotic genes, contain exons and introns (which are ultimately spliced out or
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This note was uploaded on 03/12/2011 for the course BIO 7.012 taught by Professor Lander during the Fall '10 term at MIT.

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7.012_Section_12-solution_key-2010 - Solution key - 7.012...

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