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PS_2 - Biology 115 Winter 2011 Homework Problem Set#2 1 You...

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Biology 115, Winter 2011 Homework Problem Set #2 1. You clone the ORF of a gene from a human cDNA expression library from a cancer cell line. a) How could you see if the predicted amino acid sequence of this protein resembles other proteins encoded by the human genome or the genomes of other eukaryotes? b) You find several (4-5) highly related proteins in each of humans, monkeys, mice and frogs. However yeast, fruit flies and worms have only one related protein. What do these observations suggest about the genes encoding these proteins? 2. Restriction Fragment Length Polymorphism (RFLP) is a characteristic used to differentiate DNA obtained from different individuals for forensics, paternity testing and genetic testing. Because different individuals have slightly different DNA sequences, for any particular gene, a restriction site will occasionally be absent in some people. The following diagram illustrates one such example in the human beta-globin gene. Arrows indicate the sites that endonuclease MstII recognizes. The starred site is missing in some of the population: 1.15 kb 0.2 kb a. Both your DNA sequencer and PCR machines are broken. How could you use Southern analysis to see which RFLP type you have if? Describe your probe.
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