PS_4 - Biology 115, Winter 2011 Problem Set #4 1. You...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Biology 115, Winter 2011 Problem Set #4 1. You perform a 2-hybrid experiment for protein domains X and Y and observe the following. Reporter gene A: Reporter gene B: Fusion protein 1: Gal4DBD-X (DBD=DNA binding domain) Fusion protein 2: Gal4AD-Y (AD=activation domain) Experiment Reporter gene Fusion protein(s) Measured Reporter Gene Activity (Units) 1 A 1 2 (± 3) 2 A 2 3 (± 3) 3 A 1+2 75 (± 12) 4 B 2 1 (± 2) 5 B 1+2 2 (± 4) 6 B None 2 (± 2) a. Provide an explanation for the observed results of experiments 1, 2, 3, 4 and 5. i.e. what characteristics can you attribute to the X and Y protein domains that can account for the observations? b. Provide an explanation for your observation in experiment 6. Does this influence your interpretation of experiments 1, 2, 4 and 5? How? 2. The regulation of transcription by RNA Pol II is complex, requiring the function of many sequence specific transcription factors and the mediator complex. In contrast, the regulation of RNA Pol I is simple, requires only
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 2

PS_4 - Biology 115, Winter 2011 Problem Set #4 1. You...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online