PS_5 - Biology 115, Winter 2011 Problem Set #5 1. You are...

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Biology 115, Winter 2011 Problem Set #5 1. You are studying the MBOC gene, which is thought to underlie the interesting observation that cell biologists have thick skin. A map of this gene (the transcription unit is indicated by the large black arrow) and surrounding DNA is shown below. Several of your brave classmates have donated several small fragments of their skin for the following experiment: On the day of the cell biology midterm you isolate nuclei from skin cells of a student taking both Eukaryotic Molecular Biology (EMB) and Cell Biology as well as from control skin cells of a student taking only EMB, a student taking only Cell Biology, and also from poor unsuspecting student in the History department. You lightly treat these nuclei with DNAse I (i.e. a low concentration for a short period of time), and then extract the genomic DNA and digest it completely with Eco RI. You separate these digested DNAs on a gel, transfer them to a membrane and probe them with the probe shown below. You also extract RNA from these cells, perform a Northern blot and probe it with the same probe. A restriction map of MBOC and the results of the Southern and Northern blots are shown below. (note: gray indicates a less intense band) a. What can you conclude from the Northern blot? b.
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This document was uploaded on 03/14/2011.

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PS_5 - Biology 115, Winter 2011 Problem Set #5 1. You are...

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