PS_6 - Biology 115, Winter 2011 Problem Set #6 1. The...

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Biology 115, Winter 2011 Problem Set #6 1. The cartoon below depicts the splicing pattern of a collagen gene from the nematode C. elegans . This gene is alternatively spliced, showing distinct splicing patterns in embryos and adults. The primary transcript for the gene contains 5 potential exons, which are represented as the boxes labeled 1-5 below. The positions of the initiator methionine codon and of stop codons are indicated. a. write out the order of exons that occur in the mRNA of the: - embryonic collagen -adult collagen b. In the embryo, the sequences of the 4 th potential exon are not represented in the mRNA. In the context of the embryo, would you say that these sequences are part of an intron? c. Which collagen protein, embryonic or adult, is larger? Why? 2. In the fruit fly, Drosophila melanogaster , the structure of the collagen genes is somewhat different. a. What is the structure of the mRNA produced by the: -embryonic collagen gene -adult collagen gene b. In the nematode, alternative splicing was due to use of completely different exons. In this case, what difference (in use or recognition of specific sequences) leads to alternative splicing? c.
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PS_6 - Biology 115, Winter 2011 Problem Set #6 1. The...

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