PS_7 - Biology 115, Winter 2011 Problem Set #7 1. You want...

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Biology 115, Winter 2011 Problem Set #7 1. You want to study the role of the 3’ UTR in post-transcriptional regulation of expression of the p53 gene. You decide to use a reporter assay in HeLa (human) cells for your studies where you will study the effects of changes in the 3’ UTR sequence on expression of a reporter gene. a. Draw the vector you will construct (the 3 ’UTR is ~200 bp long): b. You delete two ARE's in the 3’ UTR and see the following: Propose two different mechanisms of gene regulation that could explain this result. Construct ( => deletion of) Units of reporter activity #1 no deletion 100 #2 35-45 100 #3 105-115 1000 c. You perform northern analysis probing for your reporter transcript and see the following: There is no intron in the 3 ʹ UTR, so what could account for the size difference (~200 nt) observed for #3? d. Would you hypothesize that half-life of the p53 mRNA is regulated in cells? Why?
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PS_7 - Biology 115, Winter 2011 Problem Set #7 1. You want...

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