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PS_8 - Biology 115 Winter 2011 Problem Set#8 1 You have...

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Biology 115, Winter 2011 Problem Set #8 1. You have prepared an antibody that recognizes the 5' cap of human mRNAs with a high affinity. You perform an immunoprecipitation with this antibody. What sorts of molecules are likely to be present in the immunoprecipitate if you use nuclear extract for the experiment? What sorts of molecules are likely to be present in the immunoprecipitate if you use a cytoplasmic extract for the experiment? 2. To study bacterial ribosomes, researchers make use of antibiotics that inhibit ribosome function at several steps. Many of these compounds do not affect eukaryotic ribosomes. If they did, what would be present in the A and P sites of an initiating eukaryotic ribosome when the following antibiotics are present. What about an elongating ribosome? (Draw or list from the following choices: nothing, uncharged tRNA, AA-tRNA, peptidyl-tRNA, Met-tRNAi): a. Tetracycline: prevents binding of amino-acyl tRNA to A site of ribosome.
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