PS_9 and 10 - Biology 115, Winter 2011 Problem Set #9-10 1....

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Biology 115, Winter 2011 Problem Set #9-10 1. You have identified a protein you believe is important for licensing of DNA replication. Mutations in your protein cause cells to arrest before dividing, but with many copies of their DNA. After purifying the protein, you generate an antibody against it. How can you use this antibody to see if the protein is associated of origins of replication in a cell-cycle dependent manner? Remember, it is possible to arrest yeast cells at specific stages of the cell using temperature-sensitive mutants of cell-cycle control proteins. You have available strains that arrest at the G1, S, and G2 phases. What results would you expect if the protein inhibited re-use of an origin after a round of DNA replication? 2. Telomerase activity is not detectable in most human tissues, but is detectable in the germline. A. Why do these highly proliferative cells express telomerase? B. What is a likely explanation for the observation that telomerase activity is detectable in most cancer cells?
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PS_9 and 10 - Biology 115, Winter 2011 Problem Set #9-10 1....

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