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Unformatted text preview: Biology 115, Winter 2005 January 14 Problem Set #2 1. True or False. If false, give your reasoning. A. Cosmid vectors can carry larger inserts than phage vectors. B. YACs suffer from the drawback that they are low copy number vectors. C. Phage and cosmid vectors share the advantage of allowing very efficient transfer of DNA into bacteria. D. The key feature of an expression vector is its ability to be maintained in more than one type of host cell (for example both bacteria and yeast). E. In a cDNA library, every gene in the genome is represented. In a genomic DNA library, only a fraction of genes are represented. F. Polylinkers are flexible hinge regions of proteins that separate domains of protein function encoded by different exons. 2. What feature of Taq polymerase makes it ideal for use in PCR? 3. You discover a bacterial protein, ozonase, that beaks down ozone. You decide to clone the gene encoding the ozonase protein. a. You don't have any idea of what the amino acid sequence of the ozonase protein might be, but you do have a method for detecting the presence or absence of this protein in bacterial cells, and you also have a large quantity of the purified ozonase protein. How would you clone the gene encoding ozonase? What other reagents do you need for your strategy to work? b. After having cloned the ozonase gene, it occurs to you that, despite the lung-choking smog, people who live in Los Angeles appear to be quite healthy, and that therefore, there might be a human homolog of the ozonase gene. To determine if there is a human ozonase gene, you decide to probe a __________ blot containing samples of genomic DNA (cut with restriction enzymes), purified from ___________cells with a...
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This document was uploaded on 03/14/2011.
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