PS_3_older

PS_3_older - Bio 115, January 21, 2005 Problem Set #3 1....

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Bio 115, January 21, 2005 Problem Set #3 1. How does the mechanism of transcription termination by RNA Pol I differ from that used by RNA Pol II? 2. The regulation of transcription by RNA Pol II is complex, requiring the function of many sequence specific transcription factors and the mediator complex. In contrast, the regulation of RNA Pol I is simple, requires only one protein. Give a plausible explanation of the biological reasons underlying this difference. 3. What protein factor(s) determines which nuclear RNA polymerase is recruited to a particular promoter? 5. You discover that the p110 subunit of the yeast mediator complex binds the Gal4 activator. Your colleague has a mutant version of p110 that can no longer bind Gal4. What mutant phenotypes (i.e. observable defects) do you expect this mutation in p110 to cause? Why? 6. Why is it advantageous for a sequence specific DNA binding protein to interact with DNA in the major groove? 7. Protein kinases may regulate transcript elongation by RNA polymerase II by phosphorylating: a. the 3' hydroxyl group of the nascent transcript b. residues in the DNA template c. the C-terminal repeated domain of the largest subunit of RNA Pol. II d. TBP
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Bio 115, January 21, 2005 Problem Set #3 8. Transcription begins with location of the promoter of the gene that will be transcribed. For RNA polymerase II, this
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 4

PS_3_older - Bio 115, January 21, 2005 Problem Set #3 1....

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online