PS_4_older - Bio 115 February 4 2005 Problem Set#4 1 If you...

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Bio 115, February 4, 2005 Problem Set #4 1. If you want to differentiate between euchromatin and heterochromatin under an electron microscope, what stain would you use? In which of these regions does Pol II transcription occur? 3. In chromatin, what determines the width of the 10 nm fiber? 4. How do micrococcal nuclease and DNAse I differ in their ability to digest DNA that is assembled into chromatin? How are these different activities used to study nucleosome structure and positioning? 5. What is a chromatin remodeling machine (CRM)? How can a CRM effect transcription? Do CRM’s consume energy when performing their function? 6. Name two strategies used by cells to manipulate chromatin structure to alter transcription. 7. When in vitro transcription reactions are carried out using DNA templates that have been assembled into nucleosomes, purified transcription factors and chromatin remodeling machines, transcription can initiate-but only short transcripts are produced. This observation has led to the proposal that there must be some factor(s) in cells that allows RNA Pol II to transcribe through nucleosomes. Propose a strategy for identifying this factor.
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Bio 115, February 4, 2005 Problem Set #4 8. You are studying the MBOC
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