PS_6_older

PS_6_older - Bio 115, spring 2005 Problem set #6 1. You...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Bio 115, spring 2005 Problem set #6 - 1 - 1. You have prepared an antibody that recognizes the 5' cap of human mRNAs with a high affinity. You prepare a cell lysate and perform an immunoprecipitation. What sorts of molecules are likely to be present in the immunoprecipitate? 2. How is the 5' cap important for mRNA stability? For translation? 3. Where in the cell does capping of mRNAs occur? 4. What protein(s) binds the 3' end of mRNAs? 5. Besides alternative splicing, how can a mRNA be used to generate multiple different proteins? 6. What are some of the major differences between translation in prokaryotes and eukaryotes? 7. What are some of the energy dependent steps in translation?
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Bio 115, spring 2005 Problem set #6 - 2 - 8. How do eukaryotes determine where to initiate translation? How can this be proved experimentally? 9. How does the peptidyl transferase reaction of translation procede? In particular, a) does protein synthesis procede from the amino-terminus or carboxy-terminus of the protein? b) does the tRNA in the A site receive the nascent peptide, or does it donate it’s
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 3

PS_6_older - Bio 115, spring 2005 Problem set #6 1. You...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online