PS_8_older

PS_8_older - Bio 115 Problem set #8 March 11, 2005 1. You...

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Bio 115 Problem set #8 March 11, 2005 - 1 - 1. You are studying Slater syndrome, which is associated with a progressive loss of balance, social withdrawal and shyness. Using genetic techniques, you determine that the Slater syndrome disease gene lies within a 4 megabase region of chromosome 4. You obtain a series of cosmids with overlapping inserts that cover this region. a. What term describes a series of genomic DNA clones with overlapping inserts? b. The computer programs available for predicting genes are sometimes unreliable. Thus, you decide to directly identify expressed genes from this 4 Mb region. How can you do this? 2 points c. You isolate and sequence the disease gene from normal people and also from individuals affected by Slater syndrome. The gene’s structure is shown below. In individuals with the disease, exon 2 is always missing. You also notice that several transposons occur in the introns of this gene. What is the most likely mechanism by which this exon is lost? 2 points Exon: 1 2 3 Transposon: 1 2 d. The 5’ UTR of the Slater syndrome gene is unusually long and the AUG occurs in a poor translational context. Surprisingly, the gene is translated at a high level. Furthermore, when cells are infected with a picornavirus, the gene is translated at a high rate. Provide the most reasonable
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PS_8_older - Bio 115 Problem set #8 March 11, 2005 1. You...

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