This preview shows pages 1–2. Sign up to view the full content.
This preview has intentionally blurred sections. Sign up to view the full version.View Full Document
Unformatted text preview: Ion – concentration of salt Size exclusion Need the proteins to be very different in molecular weight. Must use different size polymers Repeatable Natural Binding Affinity SDS-Page-Method of visualizing proteins-Separates proteins based on size Preparing sample-Mechanical breakdown of cell-Proteins to be analyzed is mixed with SDS-Addition of reducing agent-Addition of tracking dye Preparing the gel-more acrylamide -> more cross-linking in the polymer -> decreasing the size of pores...
View Full Document