530L_notes_lecture1

530L_notes_lecture1 - I. DNA in bacteria A. Large bacterial...

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I. DNA in bacteria A. Large bacterial chromosome 1. ds DNA 2. 4.7 million bp 3. circular 4. encodes most genes 5. genes bundled together in operons B. Plasmids 1. ds DNA 2. replicate autonomously from chromosome 3. smaller in size (~2-10 Kb) and number of genes 4. multiple copes in single bacterial cell (depending on origin of replication) C. Plasmids are naturally occurring 1. purpose = allow for genetic exchange between bacteria 2. bacteria can transfer plasmids from cell to cell via: conjugation (not relevant to our discussion in this class) or transformation : uptake of exogenous DNA by a “competent ” bacterial cell. 3. Why transfer DNA between cells? a. genetic variation/selective advantage. For example: antibiotic resistance D. In the lab 1. the tools of recombinant DNA technology exploit the natural properties of DNA and bacterial gene transfer. II. To do molecular biology, what are the minimal requirements of a plasmid? A. origin of replication
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This note was uploaded on 03/14/2011 for the course CHEM 530L taught by Professor Hogan during the Spring '09 term at UNC.

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530L_notes_lecture1 - I. DNA in bacteria A. Large bacterial...

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